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Anti-S100A1 Polyclonal Antibody (HB172014)

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Vista general
Número de catálogoHB172014
Descripción
Anti-S100A1 Polyclonal Antibody (HB172014) is a rabbit polyclonal antibody detecting S100A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human S100A1 (Met1-Ser94).
Objetivo S100A1, Protein S100-A1, S-100 protein alpha chain, S100 calcium-binding protein A1, S100A, S-100 protein subunit alpha
Purificación Purified by antigen affinity column.
Número de acceso P23297
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein S100-A1 (S100A1) is a ~10 kDa protein. Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.

1. Kiewitz, R. et al. (2003) Biochemical and biophysical research communications 306, 550-7. PMID: 12804600
2. Nowakowski, M. et al. (2013) Biochemistry 52, 1149-59. PMID: 23351007
3. Yamaguchi, F. et al. (2012) The Journal of biological chemistry 287, 13787-98. PMID: 22399290
4. Most, P. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13889-94. PMID: 11717446
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-S100A1 Polyclonal Antibody

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