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Anti-S100A11 Polyclonal Antibody (HB147014)

Anti-S100A11 Polyclonal Antibody
Anti-S100A11 Polyclonal Antibody
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概要
カタログ番号HB147014
説明
Anti-S100A11 Polyclonal Antibody (HB147014) is a rabbit polyclonal antibody detecting S100A11 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human S100A11 (Met1-Thr105).
ターゲット MLN70, Calgizzarin, S100C, MLN 70, Protein S100-C, S100A11, S100 calcium-binding protein A11, Protein S100-A11, Metastatic lymph node gene 70 protein
精製 Purified by antigen affinity column.
アクセッション番号 P31949
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Protein S100-A11 (S100A11) is a ~11 kDa protein. Facilitates the differentiation and the cornification of keratinocytes.

注意事項 For research use only.
イメージ
  • Anti-S100A11 Polyclonal Antibody

    Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting S100A11 (HB147014) at a concentration of 27 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of S100A11 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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