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Anti-S100B Polyclonal Antibody (HY256014)

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Vista general
Número de catálogoHY256014
Descripción
Anti-S100B Polyclonal Antibody (HY256014) is a rabbit polyclonal antibody detecting S100B in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human S100B (Met1-Glu92).
Objetivo S100 calcium-binding protein B, S100B, S-100 protein subunit beta, Protein S100-B, S-100 protein beta chain
Purificación Purified by antigen affinity column.
Número de acceso P04271
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein S100-B (S100B) is a ~10 kDa protein. Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation. Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue.

1. Ostendorp, T. et al. (2011) Biochimica et biophysica acta 1813, 1083-91. PMID: 20950652
2. Baudier, J. et al. (1984) Biochimica et biophysica acta 790, 164-73. PMID: 6487634
3. Gilquin, B. et al. (2010) Molecular and cellular biology 30, 2724-36. PMID: 20351179
4. Yamaguchi, F. et al. (2012) The Journal of biological chemistry 287, 13787-98. PMID: 22399290
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-S100B Polyclonal Antibody

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