Anti-SARS-CoV-2 RBD Recombinant Nanobody (H11-H4) (VK565253)

Name: Anti-SARS-CoV-2 RBD Recombinant Nanobody (H11-H4)
Brand: abinScience
SKU: VK565253
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK565253

Description

Anti-SARS-CoV-2 RBD Nanobody (H11-H4) [H11-H4] (VK565253) is a alpaca monoclonal antibody detecting RBD in ELISA, Neutralization.

Reactivité des espèces

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Applications

ELISA, Neutralization

Espèce hôte

Alpaca

Clonalité

Monoclonal

Isotype

VHH-8His-Cys-tag

ID de clone

H11-H4

Cible

Spike glycoprotein, Spike, S glycoprotein, RBD

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Numéro d'accès

P0DTC2

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-SARS-CoV-2 RBD Nanobody (H11-H4)

Reférences

Fiche technique

Anti-SARS-CoV-2 RBD Recombinant Nanobody (H11-H4).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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