Anti-SARS1 Polyclonal Antibody (HA659014)

Name: Anti-SARS1 Polyclonal Antibody
Brand: abinScience
SKU: HA659014
Price: 168 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD): --

スペック:

50ug
100ug

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HA659014

説明

Anti-SARS1 Polyclonal Antibody (HA659014) is a rabbit polyclonal antibody detecting cytoplasmic; 6.1.1.11; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase; SARS1; SARS; SERS in ELISA, IHC, WB. Suitable for Human, Bovine, Rabbit, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant human SARS1 (Ser142-Lys472).

ターゲット

Serine--tRNA ligase, cytoplasmic; 6.1.1.11; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase; SARS1; SARS; SERS

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

P49591, Q9GMB8, P13642, P26638, Q6P799, Q6DRC0

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine--tRNA ligase, cytoplasmic (SARS1) is a ~58 kDa protein. Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.

1. Xu, X. et al. (2012) Nature communications 3, 681. PMID: 22353712

2. Xu, X. et al. (2013) Structure (London, England : 1993) 21, 2078-86. PMID: 24095058

3. Wang, C. et al. (2015) Nucleic acids research 43, 10534-45. PMID: 26433229

4. Musante, L. et al. (2017) Human mutation 38, 621-636. PMID: 28236339

5. Ravel, JM. et al. (2021) Human mutation 42, 1576-1583. PMID: 34570399

6. Verdura, E. et al. (2022) Journal of medical genetics 59, 1227-1233. PMID: 36041817

7. Vincent, C. et al. (1997) European journal of biochemistry 250, 77-84. PMID: 9431993

8. Shi, Y. et al. (2014) eLife 3, e02349. PMID: 24940000

9. Herzog, W. et al. (2009) Circulation research 104, 1260-6. PMID: 19423847

10. Fukui, H. et al. (2009) Circulation research 104, 1253-9. PMID: 19423848

注意事項

For research use only

イメージ

参考文献

データシート

Anti-SARS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-SARS1 Polyclonal Antibody (HA659014)

概要

イメージ

参考文献

データシート

SDS