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Anti-SCARA5 Polyclonal Antibody (HV465014)

Anti-SCARA5 Polyclonal Antibody
Anti-SCARA5 Polyclonal Antibody
Anti-SCARA5 Polyclonal Antibody
Anti-SCARA5 Polyclonal Antibody
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Aperçu
Numéro de catalogueHV465014
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SCARA5 (Gln118-His495).
Cible SCARA5, Scavenger receptor hlg, Scavenger receptor class A member 5
Purification Purified by antigen affinity column.
Numéro d'accès Q6ZMJ2
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Scavenger receptor class A member 5 (SCARA5) is a ~53 kDa protein. Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Preferentially binds ferritin light chain (FTL) compared to heavy chain (FTH1).

Note For research use only.
Images
  • Anti-SCARA5 Polyclonal Antibody

    Western Blot

    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

    Predict MW: 43 kDa
    Observed MW: 43 kDa

  • Anti-SCARA5 Polyclonal Antibody

    Western Blot

    Rat Muscle lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

    Lane 1: Rat Muscle lysates

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

    Predict MW: 43 kDa
    Observed MW: 43 kDa

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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