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| カタログ番号 | VK022014 | ||||||||
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| 説明 |
Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014) is a rabbit polyclonal antibody detecting Capsid protein in WB, IHC, ELISA. Suitable for O'nyong-nyong virus, Sagiyama virus, Chikungunya virus, Ross river virus, and Middelburg virus.
Highlights
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| 種反応性 | Semliki Forest virus (SFV), O'nyong'nyong virus (ONNV), Chikungunya virus (CHIKV), Ross River virus (RRV), Getah virus (GETV), Mayaro virus (MAYV) | ||||||||
| アプリケーション | WB,IHC,ELISA | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Semliki forest virus/SFV Capsid (Cys119-Trp267). | ||||||||
| ターゲット | Structural polyprotein, p130, Capsid protein, EC:3.4.21.90, Coat protein, C | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 純度 | >90% as determined by SDS-PAGE. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P03315, P0DJZ6 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Structural polyprotein (Capsid) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Roussel, A. et al. (2006) Structure (London, England : 1993) 14, 75-86. PMID: 16407067 2. Melancon, P. et al. (1987) Journal of virology 61, 1301-9. PMID: 3553612 3. Skoging, U. et al. (1998) Journal of molecular biology 279, 865-72. PMID: 9642067 4. Ulmanen, I. et al. (1979) Virology 99, 265-76. PMID: 516447 5. Vonderheit, A. et al. (2005) PLoS biology 3, e233. PMID: 15954801 6. Singh, I. et al. (1992) Journal of virology 66, 7049-58. PMID: 1433506 | ||||||||
| 注意事項 | For research use only. |
Lane 1: recombinant Semliki forest virus/SFV Capsid (Catalog No: VK022012)
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