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Anti-SERPINB1 Polyclonal Antibody (HB906014)

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Aperçu
Numéro de catalogueHB906014
Description
Anti-SERPINB1 Polyclonal Antibody (HB906014) is a rabbit polyclonal antibody detecting SERPINB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SERPINB1 (Leu13-Leu252).
Cible ELANH2, Peptidase inhibitor 2, Serpin B1, SERPINB1, MNEI, M/NEI, EI, LEI, PI2, Monocyte/neutrophil elastase inhibitor, PI-2, Leukocyte elastase inhibitor
Purification Purified by antigen affinity column.
Numéro d'accès P30740
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Leukocyte elastase inhibitor (SERPINB1) is a ~42 kDa protein. Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also acts as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity.

1. Choi, YJ. et al. (2019) Nature immunology 20, 276-287. PMID: 30692621
2. Cooley, J. et al. (2001) Biochemistry 40, 15762-70. PMID: 11747453
3. Wang, L. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1319-30. PMID: 23269243
4. El Ouaamari, A. et al. (2016) Cell metabolism 23, 194-205. PMID: 26701651
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SERPINB1 Polyclonal Antibody

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