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Каталожный номерHB906014
Описание
Anti-SERPINB1 Polyclonal Antibody (HB906014) is a rabbit polyclonal antibody detecting SERPINB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human SERPINB1 (Leu13-Leu252).
Цель ELANH2, Peptidase inhibitor 2, Serpin B1, SERPINB1, MNEI, M/NEI, EI, LEI, PI2, Monocyte/neutrophil elastase inhibitor, PI-2, Leukocyte elastase inhibitor
Очистка Purified by antigen affinity column.
Номер доступа P30740
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Leukocyte elastase inhibitor (SERPINB1) is a ~42 kDa protein. Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also acts as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity.

1. Choi, YJ. et al. (2019) Nature immunology 20, 276-287. PMID: 30692621
2. Cooley, J. et al. (2001) Biochemistry 40, 15762-70. PMID: 11747453
3. Wang, L. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1319-30. PMID: 23269243
4. El Ouaamari, A. et al. (2016) Cell metabolism 23, 194-205. PMID: 26701651
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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