Anti-SF3A1 Polyclonal Antibody (HB446014)

Name: Anti-SF3A1 Polyclonal Antibody
Brand: abinScience
SKU: HB446014
Price: 96 USD
Availability: InStock

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB446014

Descripción

Anti-SF3A1 Polyclonal Antibody (HB446014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human SF3A1 (Ser690-Lys793).

Objetivo

Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

A2VDN6, Q15459, Q8K4Z5

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Splicing factor 3A subunit 1 (SF3A1) is a ~88 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-SF3A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Lncap cell lysate
Lane 5: Hela cell lysate
Lane 6: HepG2 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse lung lysate
Lane 10: Mouse testis lysate
Lane11: Rat brain lysate
Lane12: Rat lung lysate

Referencias

Datasheet

Anti-SF3A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo