Anti-SF3A3 Polyclonal Antibody (HB387014)

Name: Anti-SF3A3 Polyclonal Antibody
Brand: abinScience
SKU: HB387014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HB387014

Description

Anti-SF3A3 Polyclonal Antibody (HB387014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human SF3A3 (Met1-Leu501).

Cible

Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q12874, Q9D554

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Splicing factor 3A subunit 3 (SF3A3) is a ~58 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.

1. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

2. Nesic, D. et al. (2001) Molecular and cellular biology 21, 6406-17. PMID: 11533230

3. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

4. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

5. Chiara, MD. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6403-7. PMID: 8022796

6. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106

7. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277

Note

For research use only

Images

Reférences

Fiche technique

Anti-SF3A3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-SF3A3 Polyclonal Antibody (HB387014)

Aperçu

Images

Reférences

Fiche technique

SDS