Anti-SHMT2 Polyclonal Antibody (HW418014)

Name: Anti-SHMT2 Polyclonal Antibody
Brand: abinScience
SKU: HW418014
Price: 168 USD
Availability: InStock

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50ug
100ug

Número:

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Número de catálogo

HW418014

Descripción

Anti-SHMT2 Polyclonal Antibody (HW418014) is a rabbit polyclonal antibody detecting SHMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SHMT2 (Asn30-His504).

Objetivo

SHMT2, Serine hydroxymethyltransferase, mitochondrial, SHMT, Glycine hydroxymethyltransferase, Serine methylase

Purificación

Purified by antigen affinity column.

Número de acceso

P34897

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Serine hydroxymethyltransferase, mitochondrial (SHMT2) is a ~55 kDa protein. Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA.

1. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

2. Giardina, G. et al. (2015) The FEBS journal 282, 1225-41. PMID: 25619277

3. Morscher, RJ. et al. (2018) Nature 554, 128-132. PMID: 29364879

4. García-Cazorla, À. et al. (2020) Acta neuropathologica 140, 971-975. PMID: 33015733

5. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

6. Minton, DR. et al. (2018) Molecular cell 69, 610-621.e5. PMID: 29452640

7. Bogenhagen, DF. et al. (2008) The Journal of biological chemistry 283, 3665-3675. PMID: 18063578

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-SHMT2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-SHMT2 Polyclonal Antibody (HW418014)

Vista general

Imágenes

Referencias

Datasheet

SDS