Anti-SHMT2 Polyclonal Antibody (HW418014)

Name: Anti-SHMT2 Polyclonal Antibody
Brand: abinScience
SKU: HW418014
Price: 168 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW418014

Description

Anti-SHMT2 Polyclonal Antibody (HW418014) is a rabbit polyclonal antibody detecting SHMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SHMT2 (Asn30-His504).

Cible

SHMT2, Serine hydroxymethyltransferase, mitochondrial, SHMT, Glycine hydroxymethyltransferase, Serine methylase

Purification

Purified by antigen affinity column.

Numéro d'accès

P34897

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Serine hydroxymethyltransferase, mitochondrial (SHMT2) is a ~55 kDa protein. Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA.

1. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

2. Giardina, G. et al. (2015) The FEBS journal 282, 1225-41. PMID: 25619277

3. Morscher, RJ. et al. (2018) Nature 554, 128-132. PMID: 29364879

4. García-Cazorla, À. et al. (2020) Acta neuropathologica 140, 971-975. PMID: 33015733

5. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

6. Minton, DR. et al. (2018) Molecular cell 69, 610-621.e5. PMID: 29452640

7. Bogenhagen, DF. et al. (2008) The Journal of biological chemistry 283, 3665-3675. PMID: 18063578

Note

For research use only.

Images

Reférences

Fiche technique

Anti-SHMT2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-SHMT2 Polyclonal Antibody (HW418014)

Aperçu

Images

Reférences

Fiche technique

SDS