Anti-SHMT2 Polyclonal Antibody (HW418014)

Name: Anti-SHMT2 Polyclonal Antibody
Brand: abinScience
SKU: HW418014
Price: 168 USD
Availability: InStock

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Каталожный номер

HW418014

Описание

Anti-SHMT2 Polyclonal Antibody (HW418014) is a rabbit polyclonal antibody detecting SHMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SHMT2 (Asn30-His504).

Цель

SHMT2, Serine hydroxymethyltransferase, mitochondrial, SHMT, Glycine hydroxymethyltransferase, Serine methylase

Очистка

Purified by antigen affinity column.

Номер доступа

P34897

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Serine hydroxymethyltransferase, mitochondrial (SHMT2) is a ~55 kDa protein. Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA.

1. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

2. Giardina, G. et al. (2015) The FEBS journal 282, 1225-41. PMID: 25619277

3. Morscher, RJ. et al. (2018) Nature 554, 128-132. PMID: 29364879

4. García-Cazorla, À. et al. (2020) Acta neuropathologica 140, 971-975. PMID: 33015733

5. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

6. Minton, DR. et al. (2018) Molecular cell 69, 610-621.e5. PMID: 29452640

7. Bogenhagen, DF. et al. (2008) The Journal of biological chemistry 283, 3665-3675. PMID: 18063578

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-SHMT2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-SHMT2 Polyclonal Antibody (HW418014)

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