Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237) (VK002063)

Name: Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237)
Brand: abinScience
SKU: VK002063
Price: 126 USD
Availability: InStock

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50ug
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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK002063

Reactivité des espèces

Sindbis virus (SINV)

Applications

ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG, kappa

ID de clone

SAA2237

Système d'expression

Mammalian cells

Cible

glycoprotein E2

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>90% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P03316

RRID

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237) (abinScience Cat# VK002063, RRID:AB_3729847)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Expédition

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Structural polyprotein (glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725

2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066

3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018

4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660

5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569

6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274

7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418

8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418

9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237)

Reférences

Fiche technique

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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