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| カタログ番号 | VK002063 | ||||
|---|---|---|---|---|---|
| 種反応性 | Sindbis virus (SINV) | ||||
| アプリケーション | ELISA | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG, kappa | ||||
| クローンID | SAA2237 | ||||
| 発現システム | Mammalian cells | ||||
| ターゲット | glycoprotein E2 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >90% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P03316 | ||||
| RRID | Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237) (abinScience Cat# VK002063, RRID:AB_3729847) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. | ||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | Structural polyprotein (glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725 2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066 3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018 4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660 5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569 6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274 7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418 8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418 9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237)
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