Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237) (VK002063)

Name: Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237)
Brand: abinScience
SKU: VK002063
Price: 126 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

VK002063

Реактивность видов

Sindbis virus (SINV)

Применения

ELISA

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG, kappa

ID клона

SAA2237

Система экспрессии

Mammalian cells

Цель

glycoprotein E2

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>90% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P03316

RRID

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237) (abinScience Cat# VK002063, RRID:AB_3729847)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Доставка

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Structural polyprotein (glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725

2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066

3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018

4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660

5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569

6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274

7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418

8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418

9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237)

Ссылки

Даташит

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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