Anti-SINV Spike glycoprotein E1 Polyclonal Antibody (VK720014)

Name: Anti-SINV Spike glycoprotein E1 Polyclonal Antibody
Brand: abinScience
SKU: VK720014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

VK720014

Description

Anti-SINV Spike glycoprotein E1 Polyclonal Antibody (VK720014) is a rabbit polyclonal antibody detecting Spike glycoprotein E1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Sindbis virus (SINV)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant SINV Spike glycoprotein E1 (Tyr807-Ser1212).

Cible

Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Purification

Purified by antigen affinity column.

Numéro d'accès

P03316

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Structural polyprotein (Spike glycoprotein E1) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725

2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066

3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018

4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660

5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569

6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274

7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418

8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418

9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546

Note

For research use only.

Images

Reférences

Fiche technique

Anti-SINV Spike glycoprotein E1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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