Anti-SLC40A1 Polyclonal Antibody (HV370014)

Name: Anti-SLC40A1 Polyclonal Antibody
Brand: abinScience
SKU: HV370014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HV370014

Descripción

Anti-SLC40A1 Polyclonal Antibody (HV370014) is a rabbit polyclonal antibody detecting SLC40A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SLC40A1 (Trp127-Val321).

Objetivo

Solute carrier family 40 member 1, SLC11A3, FPN1, SLC40A1, Iron-regulated transporter 1, IREG1, Ferroportin-1

Purificación

Purified by antigen affinity column.

Número de acceso

Q9NP59

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Ferroportin (FPN1/SLC40A1) is a ~62 kDa protein. Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma.

1. Schimanski, LM. et al. (2005) Blood 105, 4096-102. PMID: 15692071

2. Madejczyk, MS. et al. (2012) Biochimica et biophysica acta 1818, 651-7. PMID: 22178646

3. Qiao, B. et al. (2012) Cell metabolism 15, 918-24. PMID: 22682227

4. Mitchell, CJ. et al. (2014) American journal of physiology. Cell physiology 306, C450-9. PMID: 24304836

5. Aschemeyer, S. et al. (2018) Blood 131, 899-910. PMID: 29237594

6. Zhang, DL. et al. (2018) Science (New York, N.Y.) 359, 1520-1523. PMID: 29599243

7. Choi, EK. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 2228-2240. PMID: 30247984

8. Billesbølle, CB. et al. (2020) Nature 586, 807-811. PMID: 32814342

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-SLC40A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Caco2 cell lysate
Lane 4: Rat liver lysate