Anti-SLC40A1 Polyclonal Antibody (HV370014)

Name: Anti-SLC40A1 Polyclonal Antibody
Brand: abinScience
SKU: HV370014
Price: 96 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

20ug
50ug
100ug

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HV370014

説明

Anti-SLC40A1 Polyclonal Antibody (HV370014) is a rabbit polyclonal antibody detecting SLC40A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human SLC40A1 (Trp127-Val321).

ターゲット

Solute carrier family 40 member 1, SLC11A3, FPN1, SLC40A1, Iron-regulated transporter 1, IREG1, Ferroportin-1

精製

Purified by antigen affinity column.

アクセッション番号

Q9NP59

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ferroportin (FPN1/SLC40A1) is a ~62 kDa protein. Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma.

1. Schimanski, LM. et al. (2005) Blood 105, 4096-102. PMID: 15692071

2. Madejczyk, MS. et al. (2012) Biochimica et biophysica acta 1818, 651-7. PMID: 22178646

3. Qiao, B. et al. (2012) Cell metabolism 15, 918-24. PMID: 22682227

4. Mitchell, CJ. et al. (2014) American journal of physiology. Cell physiology 306, C450-9. PMID: 24304836

5. Aschemeyer, S. et al. (2018) Blood 131, 899-910. PMID: 29237594

6. Zhang, DL. et al. (2018) Science (New York, N.Y.) 359, 1520-1523. PMID: 29599243

7. Choi, EK. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 2228-2240. PMID: 30247984

8. Billesbølle, CB. et al. (2020) Nature 586, 807-811. PMID: 32814342

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-SLC40A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Caco2 cell lysate
Lane 4: Rat liver lysate