Anti-SLC40A1 Polyclonal Antibody (HV370014)

Name: Anti-SLC40A1 Polyclonal Antibody
Brand: abinScience
SKU: HV370014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HV370014

Описание

Anti-SLC40A1 Polyclonal Antibody (HV370014) is a rabbit polyclonal antibody detecting SLC40A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SLC40A1 (Trp127-Val321).

Цель

Solute carrier family 40 member 1, SLC11A3, FPN1, SLC40A1, Iron-regulated transporter 1, IREG1, Ferroportin-1

Очистка

Purified by antigen affinity column.

Номер доступа

Q9NP59

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Ferroportin (FPN1/SLC40A1) is a ~62 kDa protein. Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma.

1. Schimanski, LM. et al. (2005) Blood 105, 4096-102. PMID: 15692071

2. Madejczyk, MS. et al. (2012) Biochimica et biophysica acta 1818, 651-7. PMID: 22178646

3. Qiao, B. et al. (2012) Cell metabolism 15, 918-24. PMID: 22682227

4. Mitchell, CJ. et al. (2014) American journal of physiology. Cell physiology 306, C450-9. PMID: 24304836

5. Aschemeyer, S. et al. (2018) Blood 131, 899-910. PMID: 29237594

6. Zhang, DL. et al. (2018) Science (New York, N.Y.) 359, 1520-1523. PMID: 29599243

7. Choi, EK. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 2228-2240. PMID: 30247984

8. Billesbølle, CB. et al. (2020) Nature 586, 807-811. PMID: 32814342

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-SLC40A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Caco2 cell lysate
Lane 4: Rat liver lysate