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| カタログ番号 | HV175014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-SMAD5 Polyclonal Antibody (HV175014) is a rabbit polyclonal antibody detecting SMAD5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Coturnix japonica.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SMAD5 (Gly210-Val464). | ||||||||
| ターゲット | SMAD 5, Mothers against decapentaplegic homolog 5, SMAD5, Mothers against DPP homolog 5, Smad5, SMAD family member 5, MAD homolog 5, JV5-1, MADH5, hSmad5 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q99717 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | SMAD family member 5 (SMAD5) is a ~52 kDa protein. Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. 1. Fuchs, O. et al. (2002) Blood cells, molecules & diseases 28, 221-33. PMID: 12064918 2. Haag, J. et al. (2006) Experimental cell research 312, 1153-63. PMID: 16516194 3. Nishimura, R. et al. (1998) The Journal of biological chemistry 273, 1872-9. PMID: 9442019 4. Ruiz, L. et al. (2021) Computational and structural biotechnology journal 19, 632-646. PMID: 33510867 5. Fang, Y. et al. (2017) Cell research 27, 1083-1099. PMID: 28675158 | ||||||||
| 注意事項 | For research use only. |
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