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| カタログ番号 | HF898014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-SNAI1 Polyclonal Antibody (HF898014) is a rabbit polyclonal antibody detecting SNAI1 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SNAI1 (Met1-Arg264). | ||||||||
| ターゲット | Protein sna, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, SNAH | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O95863 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Zinc finger protein SNAI1 is a ~29 kDa protein. Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits. 1. Batlle, E. et al. (2000) Nature cell biology 2, 84-9. PMID: 10655587 2. Yook, JI. et al. (2005) The Journal of biological chemistry 280, 11740-8. PMID: 15647282 3. Lin, Y. et al. (2010) The EMBO journal 29, 1803-16. PMID: 20389281 4. Lin, T. et al. (2010) Oncogene 29, 4896-904. PMID: 20562920 5. Zhang, K. et al. (2012) The EMBO journal 31, 29-43. PMID: 21952048 6. Jin, Y. et al. (2015) Cancer letters 362, 70-82. PMID: 25827072 8. Tortorici, M. et al. (2013) ACS chemical biology 8, 1677-82. PMID: 23721412 9. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638 10. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949 | ||||||||
| 注意事項 | For research use only. |
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