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Anti-SOD1 Polyclonal Antibody (HF876014)

Anti-SOD1 Polyclonal Antibody
Anti-SOD1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHF876014
Description
Anti-SOD1 Polyclonal Antibody (HF876014) is a rabbit polyclonal antibody detecting SOD1 in ELISA, IHC, WB. Suitable for Human, Callithrix jacchus, Camelus dromedarius, Cavia porcellus, and Hylobates lar.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SOD1 (Met1-Gln154).
Cible Superoxide dismutase [Cu-Zn], SOD1, hSod1, Superoxide dismutase 1
Purification Purified by antigen affinity column.
Numéro d'accès P00441
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Superoxide dismutase [Cu-Zn] (SOD1) is a ~15 kDa protein. Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

1. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062
2. Switzer, CH. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2205044120. PMID: 36630448
Note For research use only.
Images
  • Anti-SOD1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SOD1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K562 cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse liver lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat liver lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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