Anti-SP1 Polyclonal Antibody (HY593014)

Name: Anti-SP1 Polyclonal Antibody
Brand: abinScience
SKU: HY593014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY593014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SP1 (Leu260-Ala591).

Objetivo

SP1, TSFP1, Transcription factor Sp1

Purificación

Purified by antigen affinity column.

Número de acceso

P08047

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Transcription factor Sp1 is a ~80 kDa protein. Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage.

1. Ding, H. et al. (1999) The Journal of biological chemistry 274, 19573-80. PMID: 10391891

2. Yang, X. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 6611-6. PMID: 11371615

3. Milanini-Mongiat, J. et al. (2002) The Journal of biological chemistry 277, 20631-9. PMID: 11904305

4. Bonello, MR. et al. (2004) The Journal of biological chemistry 279, 2377-82. PMID: 14593115

5. Hsu, MC. et al. (2006) The Journal of biological chemistry 281, 4718-25. PMID: 16377629

6. Hung, JJ. et al. (2006) Molecular and cellular biology 26, 1770-85. PMID: 16478997

7. Zhang, Y. et al. (2006) Molecular and cellular biology 26, 6748-61. PMID: 16943418

8. Vicart, A. et al. (2006) Journal of molecular biology 364, 897-908. PMID: 17049555

9. Olofsson, BA. et al. (2007) Molecular cancer research : MCR 5, 1319-30. PMID: 18171990

10. Chuang, JY. et al. (2008) Molecular biology of the cell 19, 1139-51. PMID: 18199680

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-SP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 3: HepG2 cell lysate