Anti-SPHK1 Polyclonal Antibody (HV674014)

Name: Anti-SPHK1 Polyclonal Antibody
Brand: abinScience
SKU: HV674014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HV674014

Description

Anti-SPHK1 Polyclonal Antibody (HV674014) is a rabbit polyclonal antibody detecting SPHK1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SPHK1 (Ser148-Leu384).

Cible

SPHK, SK 1, SPK, Acetyltransferase SPHK1, Sphingosine kinase 1, SPHK1, SPK 1, SK1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9NYA1

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Sphingosine kinase 1 (SPHK1) is a ~42 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion.

1. Gijsbers, S. et al. (2002) Biochimica et biophysica acta 1580, 1-8. PMID: 11923095

2. Alvarez, SE. et al. (2010) Nature 465, 1084-8. PMID: 20577214

3. Wang, Z. et al. (2013) Structure (London, England : 1993) 21, 798-809. PMID: 23602659

4. Shen, H. et al. (2014) Nature cell biology 16, 652-62. PMID: 24929359

5. Lee, JY. et al. (2018) Nature communications 9, 1479. PMID: 29662056

6. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219

7. Adada, MM. et al. (2013) The Journal of biological chemistry 288, 27667-27679. PMID: 23935096

8. Lima, S. et al. (2017) The Journal of biological chemistry 292, 3074-3088. PMID: 28049734

Note

For research use only.

Images

Reférences

Fiche technique

Anti-SPHK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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