Anti-SQSTM1/p62 Polyclonal Antibody (HC648014)

Name: Anti-SQSTM1/p62 Polyclonal Antibody
Brand: abinScience
SKU: HC648014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HC648014

Descripción

Anti-SQSTM1/p62 Polyclonal Antibody (HC648014) is a rabbit polyclonal antibody detecting SQSTM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SQSTM1 (Met1-Val122).

Objetivo

OSIL, EBIAP, Sequestosome-1, Ubiquitin-binding protein p62, ORCA, EBI3-associated protein of 60 kDa, p60, SQSTM1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Purificación

Purified by antigen affinity column.

Número de acceso

Q13501

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Sequestosome-1 (SQSTM1/p62) is a ~47 kDa protein. Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos.

1. Seibenhener, ML. et al. (2004) Molecular and cellular biology 24, 8055-68. PMID: 15340068

2. Babu, JR. et al. (2005) Journal of neurochemistry 94, 192-203. PMID: 15953362

3. Bjørkøy, G. et al. (2005) The Journal of cell biology 171, 603-14. PMID: 16286508

4. Pankiv, S. et al. (2007) The Journal of biological chemistry 282, 24131-45. PMID: 17580304

5. Clausen, TH. et al. (2010) Autophagy 6, 330-44. PMID: 20168092

6. Matsumoto, G. et al. (2011) Molecular cell 44, 279-89. PMID: 22017874

7. Taillebourg, E. et al. (2012) Autophagy 8, 767-79. PMID: 22622177

8. Isakson, P. et al. (2013) Autophagy 9, 1955-64. PMID: 24128730

9. Liu, X. et al. (2017) The Journal of cell biology 216, 1301-1320. PMID: 28404643

10. Zaffagnini, G. et al. (2018) The EMBO journal 37. PMID: 29343546

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-SQSTM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

Referencias

Datasheet

Anti-SQSTM1/p62 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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