Anti-SQSTM1/p62 Polyclonal Antibody (HC648014)

Name: Anti-SQSTM1/p62 Polyclonal Antibody
Brand: abinScience
SKU: HC648014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HC648014

Description

Anti-SQSTM1/p62 Polyclonal Antibody (HC648014) is a rabbit polyclonal antibody detecting SQSTM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SQSTM1 (Met1-Val122).

Cible

OSIL, EBIAP, Sequestosome-1, Ubiquitin-binding protein p62, ORCA, EBI3-associated protein of 60 kDa, p60, SQSTM1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Purification

Purified by antigen affinity column.

Numéro d'accès

Q13501

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Sequestosome-1 (SQSTM1/p62) is a ~47 kDa protein. Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos.

1. Seibenhener, ML. et al. (2004) Molecular and cellular biology 24, 8055-68. PMID: 15340068

2. Babu, JR. et al. (2005) Journal of neurochemistry 94, 192-203. PMID: 15953362

3. Bjørkøy, G. et al. (2005) The Journal of cell biology 171, 603-14. PMID: 16286508

4. Pankiv, S. et al. (2007) The Journal of biological chemistry 282, 24131-45. PMID: 17580304

5. Clausen, TH. et al. (2010) Autophagy 6, 330-44. PMID: 20168092

6. Matsumoto, G. et al. (2011) Molecular cell 44, 279-89. PMID: 22017874

7. Taillebourg, E. et al. (2012) Autophagy 8, 767-79. PMID: 22622177

8. Isakson, P. et al. (2013) Autophagy 9, 1955-64. PMID: 24128730

9. Liu, X. et al. (2017) The Journal of cell biology 216, 1301-1320. PMID: 28404643

10. Zaffagnini, G. et al. (2018) The EMBO journal 37. PMID: 29343546

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SQSTM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

Reférences

Fiche technique

Anti-SQSTM1/p62 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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