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Anti-ST6GALNAC6 Polyclonal Antibody (HV241014)

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Número de catálogoHV241014
Descripción
Anti-ST6GALNAC6 Polyclonal Antibody (HV241014) is a rabbit polyclonal antibody detecting ST6GALNAC6 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ST6GALNAC6 (Val67-Thr333).
Objetivo hST6GalNAc VI, SIAT7-F, Sialyltransferase 7F, ST6GalNAcVI, SIAT7F, Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 6, GalNAc alpha-2, 6-sialyltransferase VI, ST6GalNAc VI, ST6GALNAC6
Purificación Purified by antigen affinity column.
Número de acceso Q969X2
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (ST6GALNAC6/SIAT7-F) is a ~38 kDa protein. Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc onto glycoproteins and glycolipids, forming an alpha-2,6-linkage. Produces branched type disialyl structures by transfer of a sialyl group onto the GalNAc or GlcNAc residue inside backbone core chains having a terminal sialic acid with an alpha-2,3-linkage on Gal. ST6GalNAcVI prefers glycolipids to glycoproteins, predominantly catalyzing the biosynthesis of ganglioside GD1alpha from GM1b. Besides GMb1, MSGG and other glycolipids, it shows activity towards sialyl Lc4Cer generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen. Also has activity toward GD1a and GT1b, and can generate DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside).

1. Tsuchida, A. et al. (2003) The Journal of biological chemistry 278, 22787-94. PMID: 12668675
2. Senda, M. et al. (2007) The Biochemical journal 402, 459-70. PMID: 17123352
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ST6GALNAC6 Polyclonal Antibody

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