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Anti-ST6GALNAC6 Polyclonal Antibody (HV241014)

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Aperçu
Numéro de catalogueHV241014
Description
Anti-ST6GALNAC6 Polyclonal Antibody (HV241014) is a rabbit polyclonal antibody detecting ST6GALNAC6 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ST6GALNAC6 (Val67-Thr333).
Cible hST6GalNAc VI, SIAT7-F, Sialyltransferase 7F, ST6GalNAcVI, SIAT7F, Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 6, GalNAc alpha-2, 6-sialyltransferase VI, ST6GalNAc VI, ST6GALNAC6
Purification Purified by antigen affinity column.
Numéro d'accès Q969X2
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (ST6GALNAC6/SIAT7-F) is a ~38 kDa protein. Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc onto glycoproteins and glycolipids, forming an alpha-2,6-linkage. Produces branched type disialyl structures by transfer of a sialyl group onto the GalNAc or GlcNAc residue inside backbone core chains having a terminal sialic acid with an alpha-2,3-linkage on Gal. ST6GalNAcVI prefers glycolipids to glycoproteins, predominantly catalyzing the biosynthesis of ganglioside GD1alpha from GM1b. Besides GMb1, MSGG and other glycolipids, it shows activity towards sialyl Lc4Cer generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen. Also has activity toward GD1a and GT1b, and can generate DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside).

1. Tsuchida, A. et al. (2003) The Journal of biological chemistry 278, 22787-94. PMID: 12668675
2. Senda, M. et al. (2007) The Biochemical journal 402, 459-70. PMID: 17123352
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ST6GALNAC6 Polyclonal Antibody

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