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Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20) (JN998053)

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20)
Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20)
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Spécification :
  • 50ug
  • 100ug
  • 1mg
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  • SDS

Aperçu
Numéro de catalogueJN998053
Reactivité des espècesStaphylococcus aureus
ApplicationsELISA, Neutralization, SPR
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a
ID de cloneQD20
Cible hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, AT, Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P09616, Q2G1X0
RRID

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20) (abinScience Cat# JN998053, RRID:AB_3729029)
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Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866
2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979
Note For research use only.
Images
  • Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (QD20)

    SDS-PAGE

    SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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