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Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

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Número de catálogoJN051014
Descripción
Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014) is a rabbit polyclonal antibody detecting Sortase A in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesStaphylococcus aureus
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Staphylococcus aureus srtA/Sortase A (Gln60-Lys206).
Objetivo Sortase A, 3.4.22.70, Surface protein sorting A, srtA, SAOUHSC_02834
Purificación Purified by antigen affinity column.
Número de acceso Q2FV99
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Sortase A (srtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction.

1. Mazmanian, SK. et al. (1999) Science (New York, N.Y.) 285, 760-3. PMID: 10427003
2. Ton-That, H. et al. (1999) The Journal of biological chemistry 274, 24316-20. PMID: 10446208
3. Ton-That, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 12424-9. PMID: 10535938
4. Ton-That, H. et al. (2002) The Journal of biological chemistry 277, 7447-52. PMID: 11714722
5. Kruger, RG. et al. (2004) Biochemistry 43, 1541-51. PMID: 14769030
6. Marraffini, LA. et al. (2004) The Journal of biological chemistry 279, 37763-70. PMID: 15247224
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody

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