Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014)

Name: Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody
Brand: abinScience
SKU: JN051014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

JN051014

Описание

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody (JN051014) is a rabbit polyclonal antibody detecting Sortase A in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Staphylococcus aureus

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Staphylococcus aureus srtA/Sortase A (Gln60-Lys206).

Цель

Sortase A, 3.4.22.70, Surface protein sorting A, srtA, SAOUHSC_02834

Очистка

Purified by antigen affinity column.

Номер доступа

Q2FV99

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Sortase A (srtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction.

1. Mazmanian, SK. et al. (1999) Science (New York, N.Y.) 285, 760-3. PMID: 10427003

2. Ton-That, H. et al. (1999) The Journal of biological chemistry 274, 24316-20. PMID: 10446208

3. Ton-That, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 12424-9. PMID: 10535938

4. Ton-That, H. et al. (2002) The Journal of biological chemistry 277, 7447-52. PMID: 11714722

5. Kruger, RG. et al. (2004) Biochemistry 43, 1541-51. PMID: 14769030

6. Marraffini, LA. et al. (2004) The Journal of biological chemistry 279, 37763-70. PMID: 15247224

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Staphylococcus aureus srtA/Sortase A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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