Anti-STAT1 Polyclonal Antibody (HW746014)

Name: Anti-STAT1 Polyclonal Antibody
Brand: abinScience
SKU: HW746014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW746014

説明

Anti-STAT1 Polyclonal Antibody (HW746014) is a rabbit polyclonal antibody detecting STAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human STAT1 (Met1-Arg683).

ターゲット

Signal transducer and activator of transcription 1-alpha/beta, STAT1, Transcription factor ISGF-3 components p91/p84

精製

Purified by antigen affinity column.

アクセッション番号

P42224

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Signal transducer and activator of transcription 1-alpha/beta (STAT1) is a ~87 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.

1. Rogers, RS. et al. (2003) The Journal of biological chemistry 278, 30091-7. PMID: 12764129

2. Ungureanu, D. et al. (2003) Blood 102, 3311-3. PMID: 12855578

3. DeVries, TA. et al. (2004) The Journal of biological chemistry 279, 45603-12. PMID: 15322115

4. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278

5. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746

6. Alphonse, N. et al. (2022) Cell 185, 2354-2369.e17. PMID: 35568036

7. Liu, B. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 10626-31. PMID: 9724754

8. Chen, K. et al. (2017) Cell 170, 492-506.e14. PMID: 28753426

9. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-STAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: K562 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: Hela cell lysate

参考文献

データシート

Anti-STAT1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

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アッセイキット

Anti-STAT1 Polyclonal Antibody (HW746014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ