Anti-STAT1 Polyclonal Antibody (HW746014)

Name: Anti-STAT1 Polyclonal Antibody
Brand: abinScience
SKU: HW746014
Price: 96 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

20ug
50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HW746014

Описание

Anti-STAT1 Polyclonal Antibody (HW746014) is a rabbit polyclonal antibody detecting STAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human STAT1 (Met1-Arg683).

Цель

Signal transducer and activator of transcription 1-alpha/beta, STAT1, Transcription factor ISGF-3 components p91/p84

Очистка

Purified by antigen affinity column.

Номер доступа

P42224

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Signal transducer and activator of transcription 1-alpha/beta (STAT1) is a ~87 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.

1. Rogers, RS. et al. (2003) The Journal of biological chemistry 278, 30091-7. PMID: 12764129

2. Ungureanu, D. et al. (2003) Blood 102, 3311-3. PMID: 12855578

3. DeVries, TA. et al. (2004) The Journal of biological chemistry 279, 45603-12. PMID: 15322115

4. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278

5. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746

6. Alphonse, N. et al. (2022) Cell 185, 2354-2369.e17. PMID: 35568036

7. Liu, B. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 10626-31. PMID: 9724754

8. Chen, K. et al. (2017) Cell 170, 492-506.e14. PMID: 28753426

9. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-STAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: K562 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: Hela cell lysate

Ссылки

Даташит

Anti-STAT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа