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Anti-TAF12 Polyclonal Antibody (HS849014)

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Vista general
Número de catálogoHS849014
Descripción
Anti-TAF12 Polyclonal Antibody (HS849014) is a rabbit polyclonal antibody detecting TAF12 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human TAF12 (Met1-Lys161).
Objetivo TAF2J, Transcription initiation factor TFIID subunit 12, TAFII20, TAFII20/TAFII15, TAF15, TAF12, TAFII-20/TAFII-15, Transcription initiation factor TFIID 20/15 kDa subunits
Purificación Purified by antigen affinity column.
Número de acceso Q16514
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Transcription initiation factor TFIID subunit 12 (TAF12) is a ~17 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Brand, M. et al. (1999) The Journal of biological chemistry 274, 18285-9. PMID: 10373431
3. Mengus, G. et al. (1995) The EMBO journal 14, 1520-31. PMID: 7729427
4. Hoffmann, A. et al. (1996) Nature 380, 356-9. PMID: 8598932
5. Hoffmann, A. et al. (1996) The Journal of biological chemistry 271, 18194-202. PMID: 8663456
6. Ogryzko, VV. et al. (1998) Cell 94, 35-44. PMID: 9674425
7. Vassilev, A. et al. (1998) Molecular cell 2, 869-75. PMID: 9885574
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-TAF12 Polyclonal Antibody

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