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| カタログ番号 | VK699014 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-Tat Polyclonal Antibody (VK699014) is a rabbit polyclonal antibody detecting tat in ELISA, IHC, WB.
Highlights
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| 種反応性 | Human immunodeficiency virus type 1 (HIV-1) | ||||
| アプリケーション | ELISA, IHC, WB | ||||
| 宿主種 | Rabbit | ||||
| クローン性 | Polyclonal | ||||
| アイソタイプ | IgG | ||||
| 免疫原 | E. coli - derived recombinant HIV-1 Tat (Glu2-Glu86). | ||||
| ターゲット | Protein Tat, Transactivating regulatory protein, tat | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 精製 | Purified by antigen affinity column. | ||||
| アクセッション番号 | P04608, P05908, P69698, P12509, P69697, P19552, P20893, P19553, P35965, P12511, P05906, P20879, P05907, P04610, P04326, P04612, Q73370, P05905 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | Protein Tat is a ~9 kDa protein. Transcriptional activator that increases RNA Pol II processivity, thereby increasing the level of full-length viral transcripts. Recognizes a hairpin structure at the 5'-LTR of the nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR) and recruits the cyclin T1-CDK9 complex (P-TEFb complex) that will in turn hyperphosphorylate the RNA polymerase II to allow efficient elongation. The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive. Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function. Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin. | ||||
| 注意事項 | For research use only |
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