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Anti-TBX3 Polyclonal Antibody (HT202014)

Anti-TBX3 Polyclonal Antibody
Anti-TBX3 Polyclonal Antibody
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Especificación:
  • 20ug
  • 50ug
  • 100ug
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Vista general
Número de catálogoHT202014
Descripción
Anti-TBX3 Polyclonal Antibody (HT202014) is a rabbit polyclonal antibody detecting TBX3 in ELISA, IHC, WB. Suitable for Mouse, Dog, Danio rerio, Chicken, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human TBX3 (Leu107-Thr220).
Objetivo TBX3, T-box transcription factor TBX3, T-box protein 3
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O15119
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

T-box transcription factor TBX3 is a ~79 kDa protein. Transcriptional repressor involved in developmental processes. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes. Probably plays a role in limb pattern formation. Required for mammary placode induction, and maintenance of the mammary buds during development. Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2.

1. He, Ml. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 10212-7. PMID: 10468588
2. Lingbeek, ME. et al. (2002) The Journal of biological chemistry 277, 26120-7. PMID: 12000749
3. Martin, N. et al. (2012) The EMBO journal 31, 95-109. PMID: 22002537
Nota For research use only.
Imágenes
  • Anti-TBX3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IBX3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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