Anti-TJP1 Polyclonal Antibody (HC432014)

Name: Anti-TJP1 Polyclonal Antibody
Brand: abinScience
SKU: HC432014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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SDS

Vista general

Número de catálogo

HC432014

Descripción

Anti-TJP1 Polyclonal Antibody (HC432014) is a rabbit polyclonal antibody detecting TJP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human TJP1 (Met1-Val190).

Objetivo

TJP1, ZO1, Zonula occludens protein 1, Zona occludens protein 1, Tight junction protein ZO-1, Tight junction protein 1

Purificación

Purified by antigen affinity column.

Número de acceso

Q07157

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Tight junction protein 1 (TJP1) is a ~195 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

1. Furuse, M. et al. (1994) The Journal of cell biology 127, 1617-26. PMID: 7798316

2. Fanning, AS. et al. (1998) The Journal of biological chemistry 273, 29745-53. PMID: 9792688

3. Huo, L. et al. (2011) The EMBO journal 30, 665-78. PMID: 21240187

4. Kremerskothen, J. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 505-14. PMID: 20930113

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-TJP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

Referencias

Datasheet

Anti-TJP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-TJP1 Polyclonal Antibody (HC432014)

Vista general

Imágenes

Referencias

Datasheet

SDS