Anti-TJP1 Polyclonal Antibody (HC432014)

Name: Anti-TJP1 Polyclonal Antibody
Brand: abinScience
SKU: HC432014
Price: 96 USD
Availability: InStock

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Каталожный номер

HC432014

Описание

Anti-TJP1 Polyclonal Antibody (HC432014) is a rabbit polyclonal antibody detecting TJP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human TJP1 (Met1-Val190).

Цель

TJP1, ZO1, Zonula occludens protein 1, Zona occludens protein 1, Tight junction protein ZO-1, Tight junction protein 1

Очистка

Purified by antigen affinity column.

Номер доступа

Q07157

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Tight junction protein 1 (TJP1) is a ~195 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

1. Furuse, M. et al. (1994) The Journal of cell biology 127, 1617-26. PMID: 7798316

2. Fanning, AS. et al. (1998) The Journal of biological chemistry 273, 29745-53. PMID: 9792688

3. Huo, L. et al. (2011) The EMBO journal 30, 665-78. PMID: 21240187

4. Kremerskothen, J. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 505-14. PMID: 20930113

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-TJP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

Ссылки

Даташит

Anti-TJP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-TJP1 Polyclonal Antibody (HC432014)

Обзор

Изображения

Ссылки

Даташит

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