Anti-Mouse TNF-alpha Polyclonal Antibody (MF879014)

Name: Anti-Mouse TNF-alpha Polyclonal Antibody
Brand: abinScience
SKU: MF879014
Price: 96 USD
Availability: InStock

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MF879014

Description

Anti-TNFa/TNF-alpha Polyclonal Antibody (MF879014) is a rabbit polyclonal antibody detecting TNF-alpha in ELISA, IHC, WB. Suitable for Mouse, Rat, and Peromyscus leucopus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse TNFa/TNF-alpha (Leu80-Leu235).

Applications testées

Cible

C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Numéro d'accès

P06804

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. TNFa is the therapeutic target of adalimumab (Humira).

1. Ando, Y. et al. (2015) The Journal of biological chemistry 290, 5881-92. PMID: 25586176

2. Jeong, E. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 12894-12906. PMID: 32741026

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TNF polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-12 cell lysate
Lane 2: RAW264.7 cell lysate