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| カタログ番号 | HC351014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-TOP3A Polyclonal Antibody (HC351014) is a rabbit polyclonal antibody detecting TOP3A in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human TOP3A (Lys35-Ser179). | ||||||||
| ターゲット | DNA topoisomerase 3-alpha, TOP3, TOP3A, DNA topoisomerase III alpha | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q13472 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA topoisomerase 3-alpha (TOP3A) is a ~112 kDa protein. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. 1. Martin, CA. et al. (2018) American journal of human genetics 103, 221-231. PMID: 30057030 2. Nicholls, TJ. et al. (2018) Molecular cell 69, 9-23.e6. PMID: 29290614 | ||||||||
| 注意事項 | For research use only. |
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