

| Каталожный номер | HC351014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Описание |
Anti-TOP3A Polyclonal Antibody (HC351014) is a rabbit polyclonal antibody detecting TOP3A in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
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| Реактивность видов | Human, Mouse | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Human TOP3A (Lys35-Ser179). | ||||||||
| Цель | DNA topoisomerase 3-alpha, TOP3, TOP3A, DNA topoisomerase III alpha | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | Q13472 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | DNA topoisomerase 3-alpha (TOP3A) is a ~112 kDa protein. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. 1. Martin, CA. et al. (2018) American journal of human genetics 103, 221-231. PMID: 30057030 2. Nicholls, TJ. et al. (2018) Molecular cell 69, 9-23.e6. PMID: 29290614 | ||||||||
| Примечание | For research use only. |
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