Anti-TP53BP1 Polyclonal Antibody (HC342014)

Name: Anti-TP53BP1 Polyclonal Antibody
Brand: abinScience
SKU: HC342014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Datasheet
SDS

Vista general

Número de catálogo

HC342014

Descripción

Anti-TP53BP1 Polyclonal Antibody (HC342014) is a rabbit polyclonal antibody detecting TP53BP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human TP53BP1 (Ala1614-His1972).

Objetivo

TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1

Purificación

Purified by antigen affinity column.

Número de acceso

Q12888

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.

1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621

2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600

3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835

4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214

5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306

6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136

8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337

9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-TP53BP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo