Anti-TP53BP1 Polyclonal Antibody (HC342014)

Name: Anti-TP53BP1 Polyclonal Antibody
Brand: abinScience
SKU: HC342014
Price: 168 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC342014

Description

Anti-TP53BP1 Polyclonal Antibody (HC342014) is a rabbit polyclonal antibody detecting TP53BP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human TP53BP1 (Ala1614-His1972).

Cible

TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q12888

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.

1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621

2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600

3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835

4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214

5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306

6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136

8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337

9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958

Note

For research use only.

Images

Reférences

Fiche technique

Anti-TP53BP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai