Anti-TP53BP1 Polyclonal Antibody (HC342014)

Name: Anti-TP53BP1 Polyclonal Antibody
Brand: abinScience
SKU: HC342014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HC342014

Описание

Anti-TP53BP1 Polyclonal Antibody (HC342014) is a rabbit polyclonal antibody detecting TP53BP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human TP53BP1 (Ala1614-His1972).

Цель

TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1

Очистка

Purified by antigen affinity column.

Номер доступа

Q12888

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.

1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621

2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600

3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835

4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214

5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306

6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136

8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337

9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-TP53BP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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