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Каталожный номерJN038014
Описание
Anti-Treponema pallidum TPP47 Polyclonal Antibody (JN038014) is a rabbit polyclonal antibody detecting Treponema Pallidum (TP in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовTreponema pallidum
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Treponema pallidum TPP47 (Cys20-Gln434).
Цель Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574, Treponema Pallidum (TP, Syphilis)
Очистка Purified by antigen affinity column.
Номер доступа P29723
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Putative DD-carboxypeptidase TP_0574 (TPP47) is a ~47 kDa protein. A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin.

1. Deka, RK. et al. (2002) The Journal of biological chemistry 277, 41857-64. PMID: 12196546
2. Weigel, LM. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11611-5. PMID: 7972112
3. Radolf, JD. et al. (1989) Infection and immunity 57, 1248-54. PMID: 2647634
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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