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| カタログ番号 | HC498014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-TUBB3 Polyclonal Antibody (HC498014) is a rabbit polyclonal antibody detecting TUBB3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human TUBB3 (Met1-Lys450). | ||||||||
| ターゲット | Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q13509 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Tubulin beta-3 chain (TUBB3) is a ~50 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. 1. LaFrance, BJ. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 34996871 2. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685 3. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661 4. Tischfield, MA. et al. (2010) Cell 140, 74-87. PMID: 20074521 5. Shao, Q. et al. (2017) The Journal of neuroscience : the official journal of the Society for Neuroscience 37, 5620-5633. PMID: 28483977 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-TUBB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat testis lysate
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