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Обзор
Каталожный номерHY155014
Описание
Anti-TXN Polyclonal Antibody (HY155014) is a rabbit polyclonal antibody detecting TXN in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human TXN (Val2-Val105).
Цель Trx, TXN, TRX1, SASP, Hom s Trx, Thioredoxin, ATL-derived factor, ADF, TRX, Surface-associated sulphydryl protein, TRDX
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P10599
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Thioredoxin (TXN) is a ~11 kDa protein. Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

1. Limacher, A. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 389-96. PMID: 17182577
2. Glaser, AG. et al. (2008) Allergy 63, 1617-23. PMID: 19032234
3. Jacquot, JP. et al. (1990) Biochemical and biophysical research communications 173, 1375-81. PMID: 2176490
4. Mitchell, DA. et al. (2005) Nature chemical biology 1, 154-8. PMID: 16408020
5. Mitchell, DA. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 11609-14. PMID: 17606900
6. Wei, SJ. et al. (2000) Cancer research 60, 6688-95. PMID: 11118054
7. Hirota, K. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 3633-8. PMID: 9108029
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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