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| カタログ番号 | HB413014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-UBE2A Polyclonal Antibody (HB413014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A in ELISA, IHC, WB. Suitable for Human, Mouse, Rabbit, Rat, and Bovine.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant human UBE2A (Met1-Cys152). | ||||||||
| ターゲット | Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P49459, Q9Z255, P63146, P63148, P63149, P63147, Q32P99, P25153, P52478 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Ubiquitin-conjugating enzyme E2 A (UBE2A) is a ~17 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. 1. Kim, J. et al. (2005) Molecular cell 20, 759-70. PMID: 16337599 2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 3. Haddad, DM. et al. (2013) Molecular cell 50, 831-43. PMID: 23685073 4. Hong, JH. et al. (2015) Molecular & cellular proteomics : MCP 14, 674-85. PMID: 25582440 5. Haakonsen, DL. et al. (2024) Nature 626, 874-880. PMID: 38297121 6. Barnsby-Greer, L. et al. (2024) Nature structural & molecular biology 31, 351-363. PMID: 38182926 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-UBE2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: A375 cell lysate
Lane 5: SW620 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: N2A cell lysate
Lane 8: NIH3T3 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate
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